Phlebotomy: The Order of Draw
TLDRThis educational video script explains the importance of the order of draw in blood collection to prevent sample contamination. It covers the identification of blood tubes by color, the role of anticoagulants and additives, and specific tests associated with each tube type. The script provides a memory jogger 'stop light red, stay put green, light go' to help remember the order and emphasizes correct labeling procedures after blood draw, ensuring accurate and reliable test results.
Takeaways
- 🔬 The color of blood collection tubes indicates the type of test or department they are for, with a standard order of draw to prevent cross-contamination.
- 🚦 A memory jogger 'stop light red, stay put green, light go' helps in remembering the order of draw: sterile or blood culture tubes first, then light blue, red, SST, PST, green, lavender, and gray last.
- 🧪 Anticoagulants are chemicals that prevent blood clotting and are identified by the color of the tube's top; labels provide specific anticoagulant information.
- 🩸 Non-additive tubes, typically red topped, contain no additives and are used for tests where blood clotting is desired, such as serum tests.
- 🌐 Additive tubes contain substances that improve specimen quality or accelerate processing, and their specific additive or anticoagulant is indicated on the label.
- 🌀 SST (Serum Separator Tube) contains a gel to accelerate clotting, while PST (Plasma Separator Tube) includes fibrinogen, differing from SST.
- 📝 When drawing blood, the order of draw must be strictly followed, starting with sterile collection bottles or tubes, followed by specific tubes in a set sequence.
- 📋 Proper labeling of tubes is crucial, including patient's full name, date and time of collection, patient ID, date of birth, and the initials of the person who drew the blood.
- 🔄 After drawing blood, tubes should be gently inverted 3-8 times to mix, but not shaken, to avoid hemolysis which can ruin the sample.
- 📖 Understanding basic terms like anticoagulant, non-additive, and additive tubes, as well as the specific tests each tube is used for, is essential for accurate blood testing.
- 📚 For specialized or less common tests, such as tissue typing or toxicology studies, specific tubes like the yellow (ACD) or royal blue (element-free) are used, following the standard order of draw.
Q & A
Why is the order of draw important when collecting blood samples?
-The order of draw is crucial to prevent cross-contamination of samples with additives from other tubes, ensuring accurate test results.
What is the first tube to be drawn in the standard order of draw?
-The first tube to be drawn is the blood culture tube or sterile collection bottle, which requires a sterile specimen.
What color-coded tube is used for coagulation studies, and what does it contain?
-The light blue stopper tube is used for coagulation studies and contains sodium citrate.
What is the purpose of a serum separator tube (SST), and how is it different from a plasma separator tube (PST)?
-An SST contains a gel that accelerates clotting for serum tests. A PST, on the other hand, is a plasma separator tube with fibrinogen, the only difference being the presence of fibrinogen in a PST.
Which tube is used for hematology tests and what additive does it contain?
-The lavender or purple stopper tube is used for hematology tests and contains an additive called EDTA.
What type of tests are performed using a gray stopper tube, and what additive is it known to contain?
-A gray stopper tube is used for standard blood glucose level tests and contains sodium fluoride, also known as an anti-glycosidic additive.
What is the memory jogger for the order of draw, and how does it assist in recalling the correct sequence?
-The memory jogger is 'stop light red, stay put green, light go' which helps in remembering the order: sterile or blood culture bottles, light blue, red, SST, PST, green, lavender, and gray.
Why should tubes not be shaken after drawing blood, and what is the term for damaged samples caused by shaking?
-Tubes should not be shaken because it can destroy red blood cells, causing a condition known as hemolysis, which invalidates the samples for testing.
What information is required when manually labeling a blood collection tube, and in what order should it be written?
-When manually labeling, the required information includes the patient's full name, date and time of specimen collection, patient ID or MR number, patient's date of birth, and the initials of the person who drew the blood.
What is the correct procedure for labeling blood collection tubes in relation to the blood draw process?
-Blood collection tubes should always be labeled after the blood draw, and the information should be verified with the patient for accuracy.
What are some of the tests that can be performed using a PST tube, and what is the additive it contains?
-Tests that can be performed with a PST tube include chemistry studies, and the tube contains lithium heparin as an additive.
Outlines
🧪 Understanding Blood Collection Tubes and Order of Draw
This paragraph introduces the importance of recognizing different blood collection tubes by their colors, which identify the type of test or department. It explains the standard order of draw in hospitals and labs to prevent sample contamination. The paragraph also covers basic terms like 'anticoagulant' and describes the purpose of various tubes, such as SST and PST, which contain additives to improve specimen quality or accelerate processing. The video script emphasizes the necessity of following the order of draw to avoid false readings and provides a memory jogger to assist in remembering the sequence.
📋 The Order of Draw: Detailed Explanation and Labeling Guidelines
This section delves deeper into the specifics of the order of draw, explaining the priority of sterile collection bottles and tubes, followed by tubes containing sodium citrate for coagulation studies. It outlines the sequence for red stopper non-additive tubes used for serum tests and clarifies the conditions under which they might precede light blue tubes. The paragraph also discusses SST and PST tubes, their contents, and the types of tests they are used for. It highlights the importance of labeling tubes correctly with patient information after drawing blood and provides a memory aid for the order of draw, which includes the sequence of tube colors.
🔍 Advanced Blood Collection Concepts and Labeling Best Practices
The final paragraph covers advanced topics such as the use of different tubes for specialized testing like tissue typing and toxicology studies. It also provides a comprehensive guide on manually labeling tubes when printed labels are not available, emphasizing the importance of accurate patient information and the correct sequence of labeling steps. The script encourages viewers to review the material until they are familiar with the order of draw and concludes with a prompt to watch a short video for further clarification on the topic.
Mindmap
Keywords
💡Order of Draw
💡Anticoagulant
💡Non-Additive Tubes
💡Additive Tubes
💡Serum Separator Tube (SST)
💡Plasma Separator Tube (PST)
💡EDTA
💡Sodium Fluoride
💡Hemolysis
💡Labeling
💡Memory Jogger
Highlights
Color-coded blood tubes identify the type of test or department.
Standard order of draw in hospitals and labs prevents sample cross-contamination.
Memory jogger 'stop light red, stay put green, light go' aids in remembering the order of draw.
Anticoagulants are chemicals that prevent blood clotting, identified by tube color or label.
Non-additive tubes, typically red top, contain no additives and blood clots upon centrifugation.
Additive tubes contain substances that improve specimen quality or accelerate processing.
Serum separator tubes (SST) contain a gel to accelerate clotting.
Plasma separator tubes (PST) differ from SST by containing fibrinogen.
Blood culture tubes require a sterile specimen and are drawn first in the order of draw.
Coagulation studies, like PT or PTT tests, are conducted using blue stopper tubes with sodium citrate.
Red stopper non-additive tubes are used for serum tests and can act as a discarded tube when using a butterfly.
Serum separator tubes (SST) with glass particles or thexotropic gel are used for various chemistry and serum tests.
Plasma separating tubes (PST) with lithium heparin are used for chemistry tests, differing from SST by fibrinogen presence.
Green stopper tubes with sodium heparin are used for chemistry studies, including electrolytes and metabolic panels.
Purple or lavender stopper tubes with EDTA are used for hematology tests like CBC, ESR, HCT, RBC, WBC, or HGB.
Gray stopper tubes with sodium fluoride, an anti-glycosidic additive, are used for blood glucose level tests.
Yellow tubes with acid citrate dextrose are used for specialty tests like tissue typing and DNA studies.
Royal blue tubes may contain traces of heparin and are used for toxicology studies.
Proper tube labeling includes patient's name, date and time of collection, ID number, date of birth, and initials of the person who drew the blood.
Tubing should be inverted gently to mix without causing hemolysis; shaking destroys red blood cells.
Labeling should occur after drawing blood and should be verified with the patient for accuracy.
Transcripts
Browse More Related Video
Order of Draw and Additives | Blood Collection
Lecture 19: Specimen Collection & Processing I Audio
Order of sample draw | Order of draw | Blood sample collection order
What's inside of blood? | Lab values and concentrations | Health & Medicine | Khan Academy
Blood and Urine Labratory Tests for Veterinary Assistants
Blood bottles guide | UKMLA | CPSA
5.0 / 5 (0 votes)
Thanks for rating: