Order of sample draw | Order of draw | Blood sample collection order
TLDRThis educational video introduces the concept of 'Order of Draw' for blood sampling to prevent cross-contamination and test result errors. It emphasizes the importance of collecting blood in a specific sequence, starting with blood culture bottles, followed by coagulation and nonadditive tubes, and ending with additive tubes. The video also provides a mnemonic 'Boys like red, girls like gray' to remember the order and explains the purpose and process of inverting various tubes, including their colors and the number of inversions required.
Takeaways
- 🔬 The 'Order of Draw' is a protocol to avoid cross-contamination and test result errors when withdrawing multiple blood samples.
- 📍 The specific order of drawing blood is crucial to prevent additive carryover that can contaminate subsequent samples.
- 🧪 Blood samples should be collected in tubes with a recommended sequence starting with blood culture bottles and ending with additive tubes.
- 💉 The term 'reflex' illustrates how chemicals can move from one tube to another through the needle, contaminating the sample.
- 🔴 The mnemonic 'Boys Like Red, Girls Like Gray' helps to remember the order of drawing blood into different colored tubes.
- 🏺 The first tube in the order is the blood culture bottle, which requires 8 to 10 inversions to mix the sample properly.
- 🔵 The light sky blue tube containing citrate follows, needing 3 to 4 inversions.
- 🔺 Red top tubes, also known as serum tubes, require 8 to 10 inversions, and there are also serum gel tubes in gold or yellow.
- 🟢 Green top tubes, or Heparin tubes, also necessitate 8 to 10 inversions for proper mixing.
- 🟪 Lavender top tubes, known as EDTA tubes, follow the same inversion protocol as Heparin tubes.
- ⬜ Gray top tubes, containing fluoride oxalate, end the sequence and also require 8 to 10 inversions.
- 📝 The video provides a reference for different tubes used in blood collection, including their colors, additives, and the number of inversions needed.
Q & A
What is the main topic of the video?
-The main topic of the video is the order of draw for withdrawing multiple blood samples to avoid cross contamination and test result errors.
Why is it important to follow the order of draw when collecting blood samples?
-Following the order of draw is important to prevent possible test result errors due to additive carryover and to avoid cross contamination between samples.
What is the term used to describe the process where a chemical from one tube can contaminate another?
-The process is called 'reflex', where the chemical can move from one tube and enter the needle, contaminating the next tube when it is activated.
What is the recommended first step in the order of draw?
-The first step in the order of draw is to collect the blood in a blood culture bottle.
What is the second step in the order of draw after the blood culture bottle?
-The second step is to collect the blood in a coagulation tube.
What type of tube is recommended to be drawn last in the order of draw?
-The last tubes to be drawn are the additive tubes, which also have a specific order for collection.
Can you explain the mnemonic 'boys like red, girls like gray' in the context of the order of draw?
-The mnemonic helps to remember the order of draw for additive tubes: it suggests that boys (referring to serum tubes) like red, and girls (referring to EDTA tubes) like gray, indicating the colors of the tubes to be drawn last.
What is the inversion time for a blood culture tube?
-The inversion time for a blood culture tube is 8 to 10 times.
What does the light sky blue tube contain and what is its inversion time?
-The light sky blue tube contains citrate and its inversion time is 3 to 4 times.
What is the color of the tubes used for serum collection and what is the inversion time for these tubes?
-Serum collection tubes are red, gold, or yellow in color, and the inversion time is 8 to 10 times.
What is the purpose of the inversion of blood collection tubes?
-The purpose of inverting the blood collection tubes is to mix the blood with the additive or anticoagulant present in the tube to prevent clotting or other reactions that could affect the test results.
What are the different types of tubes mentioned in the script and their respective caps or colors?
-The different types of tubes mentioned are blood culture tubes (no specific color mentioned), sodium citrate tubes with blue caps, serum tubes with red, gold, or yellow caps, Heparin tubes with dark green or light green caps, EDTA tubes with lavender caps, and fluoride oxalate tubes with gray caps.
Outlines
🔬 Introduction to Order of Draw in Blood Sampling
This paragraph introduces the concept of 'Order of Draw' in the context of blood sampling to avoid cross-contamination and test result errors. It emphasizes the importance of collecting blood samples in a specific order to prevent additive carryover. The speaker invites viewers to subscribe to the channel for updates and begins discussing the order of draw, its purpose, and the process of reflex contamination that can occur if samples are not drawn correctly.
📝 The Purpose and Process of Order of Draw
The paragraph explains the purpose of the order of draw, which is to prevent errors in test results due to additive carryover and cross-contamination. It describes the process of reflex, where chemicals from one tube can contaminate the next sample if not drawn in the correct order. The paragraph also outlines the types of vacutainers and their respective chemicals, such as sodium citrate, fluoride, and heparin, and provides the recommended order of draw for blood samples.
📚 Recommended Order of Draw and Mnemonic
This section provides the recommended order for drawing blood samples, starting with blood culture bottles, followed by coagulation tubes, nonadditive tubes, and finally additive tubes. A mnemonic is introduced to help remember the order: 'Boys like red, girls like gray.' The paragraph also lists the specific colors of the collection tubes, the chemicals they contain, and the number of inversions required for each type of tube to ensure proper mixing and prevent sample contamination.
🌈 Color Coding and Inversion Times for Blood Collection Tubes
The paragraph delves into the color coding of blood collection tubes and the specific number of inversions needed for each type. It lists the blood culture tube, light sky blue tube with citrate, red serum tube, green heparin tube, lavender EDTA tube, and gray fluoride oxalate tube, detailing the chemical content and the recommended inversion times for each. This information is crucial for ensuring accurate blood sample collection and analysis.
🏥 Institutional Policies and Closing Remarks
The final paragraph reminds viewers that the order of draw may vary according to institutional policies and encourages them to follow the guidelines provided by their respective organizations. The speaker also invites viewers to like the video and subscribe to the channel for instant notifications, concluding with a thank you and well-wishes for the viewers.
Mindmap
Keywords
💡Order of Draw
💡Cross-contamination
💡Blood Culture
💡Coagulation Tube
💡Nonadditive Tube
💡Additive Tubes
💡Carryover
💡Reflex
💡Sodium Citrate
💡Inversion
💡Color-coded Tubes
Highlights
Introduction to the concept of 'order of draw' for blood samples to avoid cross-contamination.
Explanation of the necessity for a specific order of drawing blood samples to prevent test result errors due to additive carryover.
Description of the 'reflex' process, illustrating how chemicals can move from one tube to another, causing contamination.
Listing of different chemicals contained in vacutainers such as sodium citrate, fluoride, and heparin.
Recommended order of draw starting with blood culture bottles, followed by coagulation tubes, nonadditive tubes, and finally additive tubes.
Mnemonic 'boys like red, girls like gray' to remember the order of draw for blood collection tubes.
Identification of the colors of collection tubes and the chemicals they contain, such as light blue top tubes for citrate.
Inversion times for blood culture tubes, which should be inverted 8 to 10 times.
Details on the light sky blue tube containing citrate and its inversion time of 3 to 4 times.
Information on red top serum tubes and their inversion time, also mentioning serum gel tubes in gold or yellow.
Description of green top Heparin tubes and their required inversion time of 8 to 10 times.
Explanation of lavender top Ethylene Diamine Tetraacetic Acid (EDTA) tubes and their inversion time.
Details on gray top fluoride oxalate tubes and their inversion time, also mentioning other types of tubes used in blood collection.
Emphasis on the importance of following institutional policies for the order of draw in blood sample collection.
Encouragement for viewers to like, subscribe, and hit the Bell icon for instant notifications on the channel.
Conclusion and thanks for watching, wishing viewers a nice day.
Transcripts
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