Pathology practicals I INSTRUMENTS in Pathology I part 1 I Identification, Uses.

ilovepathology
9 Jun 202108:06
EducationalLearning
32 Likes 10 Comments

TLDRThis tutorial from Tyler Pathology offers an introductory guide to identifying and understanding the various instruments used in pathology, essential for students. The video script covers instruments like the albuminometer, urinometer, and vacutainer tubes, highlighting their specific uses, such as quantifying albumin in urine, measuring specific gravity, and collecting blood for different tests. It also introduces tools for bone marrow aspiration, liver biopsy, and hemoglobin estimation, promising more detailed descriptions in upcoming videos.

Takeaways
  • πŸ˜€ The tutorial is from 'Pathology Made Simple' and focuses on identifying instruments used in pathology.
  • πŸ” The detailed description of each instrument will be covered in a separate video, while this tutorial provides a basic overview.
  • πŸ§ͺ The S. Back's Albuminometer is used to estimate albumin in urine quantitatively, with markings for reagent and urine.
  • 🌑 The Uranometer measures the specific gravity of urine, with calibrations from 1.000 to 1.060.
  • 🩸 The Weindrop's Tube is used for determining packed cell volume, hematocrit, ESR, and preparing buffy coat.
  • πŸ’‰ The Disposable LP Needle is used for lumbar puncture, with diagnostic and therapeutic indications including CSF analysis and spinal anesthesia.
  • 🩸 Vacutainer tubes are color-coded for different uses, such as coagulation studies (blue), glucose estimation (gray), serological investigations (red), bone marrow studies (green), and hematological investigations (lavender).
  • πŸ“ The Westergren Pipette is used to estimate erythrocyte sedimentation rate, with markings from 0 to 200.
  • πŸ” The Neubauer Chamber is used for counting cells, RBC, WBC, platelets, and spermatozoa in semen analysis.
  • 🩺 Saha's Bone Marrow Aspiration Needle is adjustable for depth, used for diagnosing conditions like anemia, leukemia, and fever of unknown origin.
  • πŸ’‰ The Sahli's Hemoglobinometer is used for estimating hemoglobin by the acid hematin method, with specific markings for percentage and grams percentage.
Q & A
  • What is the purpose of the tutorial from 'Pathology Made Simple'?

    -The tutorial aims to help students identify various instruments used in pathology and understand their uses, particularly those that might be encountered in examinations.

  • What is the specific use of the S. Back's Albuminometer?

    -The S. Back's Albuminometer is used to estimate albumin in urine quantitatively, with markings to measure from 0 to 12 grams.

  • Can you describe the parts of a urinometer?

    -A urinometer consists of three parts: the lower end called the 'weight', the middle part called the 'float', and the upper part called the 'stem' which has calibrations to measure specific gravity.

  • What is the range of specific gravity that a urinometer measures?

    -A urinometer measures specific gravity ranging from 1.000 to 1.060.

  • What is the purpose of the Wintrobe's tube?

    -The Wintrobe's tube is used for the determination of packed cell volume or hematocrit, determination of ESR, and also for the preparation of buffy coat.

  • What are the indications for using a lumbar puncture needle?

    -A lumbar puncture needle is used for diagnostic indications such as the analysis of cerebrospinal fluid (CSF) during myelography, and therapeutic indications like spinal anaesthesia and treatment with chemotherapy or antibiotics.

  • How are Vacutainer tubes categorized and what are their uses?

    -Vacutainer tubes are color-coded and used for collecting blood for various investigations: blue for coagulation studies, gray for glucose estimation, red for serological investigations, and green for bone marrow studies.

  • What is the function of the Westergren pipette?

    -The Westergren pipette is used to estimate the erythrocyte sedimentation rate, with markings from 0 to 200 on the next 20 centimeters of its length.

  • What is the purpose of the Neubauer's chamber?

    -The Neubauer's chamber is used for counting cells, red blood cells (RBC), white blood cells (WPC), platelets, and also for counting spermatozoa in semen analysis.

  • What are the parts of Saha's bone marrow aspiration needle and its main indication?

    -Saha's bone marrow aspiration needle consists of a trocar (cannula) and an adjustable side guard for depth control. Its main indication is in the diagnosis of conditions like megaloblastic anemia, leukemias, idiopathic thrombocytopenic purpura, multiple myelomas, and fever of unknown origin.

  • What is the use of the Sahli's hemoglobin set and how is it different from other pipettes?

    -The Sahli's hemoglobin set is used for estimating hemoglobin by the acid hematin method. It includes a special hemoglobin pipette without a central bulb and markings for 20 cubic centimeters, distinguishing it from other pipettes.

  • What are the components of the Vim-Silverman liver biopsy needle and its main uses?

    -The Vim-Silverman liver biopsy needle consists of a distillate, a cannula, and a bifid needle with a sharp cutting edge. It is used for performing liver biopsies to evaluate jaundice, cirrhosis, storage disorders, and for diagnosing various liver tumors.

Outlines
00:00
πŸ” Pathology Instruments Identification Guide

This paragraph introduces the tutorial's aim to teach students how to identify various instruments used in pathology and their uses. It covers the albuminometer for quantitative estimation of albumin in urine, the uranometer for measuring urine's specific gravity, the windrops tube for packed cell volume and ESR, and the vacutainer tubes for blood collection with different anticoagulants. Also mentioned are the disposable LP needle for lumbar puncture, the Westergren pipette for erythrocyte sedimentation rate, and the Neubauer chamber for cell counting. The paragraph concludes with the Sahala's bone marrow aspiration needle, emphasizing its adjustable side guard for safe penetration depth.

05:01
🧬 Hematology and Liver Biopsy Instrumentation

The second paragraph focuses on the instruments used for hemoglobin estimation, including the Sahli's apparatus, hemoglobinometer, and the acid hematin method. It also discusses the liver biopsy needle with its parts and uses for diagnosing liver-related conditions. The paragraph provides a brief overview of the solid hemoglobin set, the Vim Silverman liver biopsy needle, and its indications for liver evaluation. It concludes with an invitation for viewers to stay tuned for more detailed descriptions and procedural details in upcoming videos, and encourages interaction through likes, comments, and subscriptions.

Mindmap
Keywords
πŸ’‘Albuminometer
An albuminometer is a laboratory instrument used to estimate the amount of albumin in urine. In the video, it is described as a tube with specific markings for reagent and urine, and graduations from 0 to 12 grams. It is crucial for quantitative estimation of albumin in urine, which is a key test in diagnosing various kidney diseases.
πŸ’‘Uranometer
A uranometer is an instrument designed to measure the specific gravity of urine. It consists of three parts: a weight, a float, and a stem with calibrations. The specific gravity ranges from 1.000 to 1.060. This tool is essential in pathology for assessing kidney function and detecting conditions like diabetes.
πŸ’‘Wintrobe Tube
The Wintrobe tube is a specific type of tube used in hematology. It is closed at one end and open at the other, with graduations from 10 to 0 and vice versa. This tube is used for determining packed cell volume or hematocrit, which is a measure of the proportion of red blood cells in the blood. It is also used for measuring erythrocyte sedimentation rate (ESR) and preparing buffy coat.
πŸ’‘Lumbar Puncture Needle
A lumbar puncture needle, also known as a spinal tap needle, is used to collect cerebrospinal fluid (CSF) from the spinal column. The video mentions it as a stainless steel needle with a stylet inside. It is used for diagnostic purposes, such as analyzing CSF during myelography, and therapeutic purposes like spinal anesthesia and chemotherapy.
πŸ’‘Vacutainer Tubes
Vacutainer tubes are color-coded plastic tubes used for collecting blood samples for various tests. Each color-coded cap indicates a specific anticoagulant or additive, such as sodium citrate for coagulation studies, sodium fluoride for glucose estimation, and no anticoagulant for serological investigations. These tubes are essential in pathology for conducting a wide range of blood tests.
πŸ’‘Westergreen Pipette
The Westergren pipette is a laboratory tool used for measuring the erythrocyte sedimentation rate (ESR). It is a long pipette with both ends open and specific markings from 0 to 200. The video mentions it in the context of estimating ESR, which is a common test to assess inflammation and other conditions.
πŸ’‘Neubauer Chamber
The Neubauer chamber is a specialized slide used for counting cells, such as red blood cells, white blood cells, platelets, and spermatozoa. It has a counting grid with a specific area for accurate cell counting. The video describes it as an improved version with clear lines, making it easier to perform precise cell counts.
πŸ’‘Bone Marrow Aspiration Needle
A bone marrow aspiration needle is used to collect bone marrow samples for diagnostic purposes. The video describes it as having a trocar, cannula, and an adjustable side guard for controlling the depth of penetration. This tool is crucial for diagnosing conditions like anemia, leukemia, and other bone marrow disorders.
πŸ’‘Hemoglobin
Hemoglobin is a protein in red blood cells that carries oxygen. The video discusses the estimation of hemoglobin levels using various instruments like the Sahli's hemoglobinometer and the acid hematin method. Hemoglobin levels are critical in diagnosing anemia and other blood disorders.
πŸ’‘Liver Biopsy Needle
A liver biopsy needle is used to obtain tissue samples from the liver for diagnostic purposes. The video describes the Vim Silverman liver biopsy needle, which has a specific design with a beveled edge and a bifid needle for cutting and retaining tissue. Liver biopsies are performed to evaluate conditions like jaundice, cirrhosis, and liver tumors.
Highlights

Introduction to identifying instruments in pathology and their uses.

Description of the S. Back's Albuminometer for quantitative estimation of albumin in urine.

Explanation of the Urinometer's parts and its use in measuring urine's specific gravity.

Introduction to the Winthrop Tube for determining packed cell volume, hematocrit, ESR, and preparing buffy coat.

Discussion on the use of disposable LP needles for lumbar puncture in diagnostic and therapeutic procedures.

Description of Vacutainer tubes and their color-coded system for various blood investigations.

Explanation of the Westergren Pipette for estimating erythrocyte sedimentation rate.

Introduction to the Neubauer Chamber for cell counting.

Description of Saha's Bone Marrow Aspiration Needle and its adjustable side guard for depth control.

Discussion on the uses of bone marrow aspiration in diagnosing various blood disorders.

Introduction to the complete set of Sahli's Hemoglobin apparatus and its components.

Explanation of the acid hematin method for estimating hemoglobin using Sahli's apparatus.

Introduction to the Vim-Silverman Liver Biopsy Needle and its parts.

Discussion on the indications for liver biopsy using the Vim-Silverman needle.

Upcoming detailed descriptions of each instrument and procedural details in future videos.

Encouragement to like, comment, subscribe, and share the video for more pathology content.

Transcripts
Rate This

5.0 / 5 (0 votes)

Thanks for rating: