Part-3 Types of chromatography on the basis of principle || Adsorption & Partition chromatography

MJD Chemistry
17 Jan 202111:40
EducationalLearning
32 Likes 10 Comments

TLDRThe provided script discusses various aspects of chromatography techniques, including types like partition, adsorption, and ion exchange chromatography. It explains the process of separating substances using different stationary and mobile phases, and highlights the importance of understanding the properties of the sample and the chromatography system. The script also touches on the practical steps involved in setting up and conducting chromatography experiments, emphasizing the need for precision and the impact of technique on results.

Takeaways
  • 😀 The script discusses a process involving 'swimming chromatography' and its types, suggesting a technical or scientific context.
  • 🔍 It mentions 'partition chromatography' and its principles, indicating a focus on a specific method within chromatography.
  • 📊 The transcript refers to various options and settings in chromatography, such as 'electronic oil exchange chromatography', which may relate to advanced techniques.
  • 🧪 There is mention of 'phosphate' and 'exploitation size', which could be related to chemical components or parameters in the chromatographic process.
  • 📝 The script talks about 'phenomenon chromatography', which might be a specific observation or phenomenon observed during the process.
  • 🔬 There is a discussion about the properties of 'type of matter' in chromatography, possibly referring to solid or liquid states.
  • 📈 The transcript includes steps or stages in the process, such as 'first station' and 'second station', indicating a sequential method.
  • 📝 It mentions 'graph' and 'graphic' multiple times, which might refer to visual representations or data analysis in chromatography.
  • 🔑 The term 'partition' is repeatedly used, emphasizing its importance in the chromatographic process described.
  • 📋 The script also touches on the differences in fees or costs associated with different types of chromatography, suggesting a practical or economic aspect.
  • 🔍 Lastly, the transcript seems to describe a setup or apparatus for chromatography, including components like 'stoppers' and 'silica gel'.
Q & A

  • What is the primary topic discussed in the video transcript?

    -The primary topic discussed in the video transcript is different types of chromatography techniques.

  • How many types of chromatography are mentioned in the script?

    -Five types of chromatography are mentioned: partition chromatography, adsorption chromatography, ion exchange chromatography, size exclusion chromatography, and affinity chromatography.

  • What is partition chromatography?

    -Partition chromatography is a type of chromatography where the stationary phase is a liquid film on a solid support and the separation is based on partitioning of the analyte between the mobile phase and the stationary phase.

  • What is the main difference between partition chromatography and adsorption chromatography?

    -The main difference is that partition chromatography involves a liquid stationary phase, while adsorption chromatography involves a solid stationary phase.

  • How is paper chromatography related to partition chromatography?

    -Paper chromatography is a type of partition chromatography where the stationary phase is water trapped in the fibers of the paper and the mobile phase moves through it by capillary action.

  • What is the role of water in paper chromatography?

    -In paper chromatography, water acts as the stationary phase, absorbed into the paper fibers, and helps in the partitioning process of the analytes.

  • What is ion exchange chromatography?

    -Ion exchange chromatography is a technique where ions are exchanged between the analyte and the stationary phase, which is typically a resin with charged groups.

  • What materials are commonly used as stationary phases in column chromatography?

    -Common materials used as stationary phases in column chromatography include silica gel and calcium carbonate.

  • What is the purpose of using a stopper in column chromatography?

    -A stopper in column chromatography is used to control the flow of the mobile phase through the column, allowing for precise separation of analytes.

  • What is the difference between adsorption and absorption in chromatography context?

    -In chromatography, adsorption refers to the process where molecules adhere to the surface of the solid stationary phase, while absorption refers to the process where molecules penetrate into the bulk of the stationary phase.

Outlines
00:00
📊 Types of Chromatography Methods

This paragraph discusses various types of chromatography methods, including partition chromatography, adsorption chromatography, ion-exchange chromatography, and size-exclusion chromatography. It highlights their definitions and basic working principles, emphasizing the differences in how each type separates components based on different physical and chemical properties.

05:02
📄 Characteristics and Use of Paper Chromatography

The paragraph elaborates on the details of paper chromatography, explaining its mechanism and the importance of water content in the paper. It describes how the stationary phase (paper) and the mobile phase (solvent) interact, leading to the separation of substances. The summary includes examples and emphasizes the significance of the paper's physical properties in the chromatographic process.

10:02
💧 Adsorption and Stationary Phases in Chromatography

This section explains adsorption chromatography, focusing on the differences between stationary phases like solid and liquid. It describes the process of how substances interact with the stationary phase and the role of solvents in facilitating these interactions. The paragraph uses examples, such as applying mehndi, to illustrate adsorption and retention mechanisms in chromatography.

Mindmap
Keywords
💡Chromatography
Chromatography is a technique used to separate mixtures into their individual components based on their affinity to the stationary phase. In the context of the video, it seems to be the main theme, discussing various types and methods, such as 'swimming chromatography' and 'electronic oil exchange chromatography'. The script mentions '25 ray' as a type, which might be a specific parameter or method within chromatography.
💡Partition Chromatography
Partition chromatography is a method where the separation of compounds is based on their distribution between two immiscible phases. The script refers to 'partition' in the context of setting up a 'partition matter of partition', suggesting it is a type of chromatography being discussed, possibly involving the separation of substances in a graphical box.
💡Adsorption Chromatography
Adsorption chromatography is a specific type of chromatography where the separation of compounds is based on their adsorption to a solid surface. The script mentions 'adsorption' in the context of a 'good job option', indicating it might be a preferred method or an option being discussed for its effectiveness in the chromatographic process.
💡Electron Exchange Chromatography
Electron exchange chromatography is not a standard term in chromatography, but it could refer to a method involving electron transfer or charge interactions during the separation process. The script mentions it as 'electronic oil exchange chromatography', which might be a specialized technique or a proprietary method being discussed.
💡Phosphatide
Phosphatides are a class of lipids that are important components of cell membranes. In the script, 'phosphatide' is mentioned in the context of 'size expression size exploitation', which could imply its role in a specific step or aspect of the chromatographic process.
💡Stationary Phase
The stationary phase in chromatography is the medium that is stationary during the process and to which the compounds in the mixture adhere. The script refers to 'stationary phase' in the context of discussing different types of chromatography, indicating its importance in the separation process.
💡Mobile Phase
The mobile phase is the component of the chromatography system that moves through the stationary phase, carrying the sample mixture. The script mentions 'mobile phase' in the context of discussing the properties and behavior of the chromatographic system, such as its interaction with the stationary phase.
💡Graph
In the context of the video, 'graph' likely refers to a graphical representation of data, such as a chromatogram, which visually displays the separation of compounds over time or distance. The script mentions 'graph' in relation to 'graphic' and 'graphic box', suggesting it is used to visualize or analyze the chromatographic process.
💡Partition Coefficient
The partition coefficient is a measure of the distribution of a compound between the stationary and mobile phases in chromatography. The script refers to 'partition coefficient' in the context of discussing the properties of the compounds being separated, indicating its importance in determining the efficiency of the separation.
💡Silica Gel
Silica gel is a common stationary phase used in chromatography, particularly in column chromatography. The script mentions 'silica gel' in the context of discussing the materials used in the chromatographic setup, suggesting it might be a component of the system being described.
💡Sample
A sample in chromatography refers to the mixture that is being separated into its individual components. The script mentions 'sample' in the context of discussing the process of separation and analysis, indicating it is the substance being examined in the video.
Highlights

Discussion on swimming chromatography type pumplkar with a principal rate of 25 rays.

Introduction to the first type of chromatography, partition chromatography.

Mention of Adams option in chromatography and its significance.

Explanation of electronic oil exchange chromatography and its process.

Differentiating between the types of chromatography based on their properties and uses.

The importance of the solid phase in solid-liquid chromatography.

Techniques for handling and preparing samples for chromatography.

The role of the stationary phase in chromatography and its impact on results.

Discussion on the properties of specific types of chromatography, such as the liquid phase.

The use of specific chemicals in chromatography and their effects on the process.

The concept of solute interaction with the stationary phase in chromatography.

The impact of temperature and pressure on the efficiency of chromatography.

The importance of selecting the right type of chromatography for specific applications.

Techniques for improving the resolution in chromatography.

The role of mobile phase in chromatography and its influence on the separation process.

Discussion on the use of silica gel in chromatography and its properties.

The process of adding solvents in chromatography and their role in sample separation.

Final thoughts on the practical applications and implications of chromatography techniques.

Transcripts

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ChromatographyAnalytical ChemistrySwimming ChromatographyType PumpPrincipalTariffLeft SideDiscusPhosphatideExpression SizeExploitation