Radial paper chromatography Experiment | Seperation of inks by radial chromatography | #experiment

MJD Chemistry
11 Apr 202104:58
EducationalLearning
32 Likes 10 Comments

TLDRThis instructional video script outlines the process of performing radial chromatography, a technique used for separating components in a mixture. It begins with the application of the sample on a filter paper, followed by cutting and chilling. The script then details the development of the chromatogram using a fielder, timing, and checking the process. It emphasizes the importance of proper sample application, the use of a mobile phase, and the final steps of solvent evaporation and component separation. The video aims to educate viewers on the intricacies of radial chromatography, highlighting its versatility and potential for various applications.

Takeaways
  • 🔬 The script discusses the use of radial chromatography, a technique used in the first impression of different components, particularly in the context of a chemistry experiment.
  • 🧪 If a student does not provide an exam, the script mentions a method to set them apart, possibly using radial chromatography as a tool for differentiation.
  • 🔍 The process involves a specific filter, which is mentioned to be completely round and is used to separate components in a cold field.
  • 📝 The script describes a method to cut and take a sample from the center of a completely round filter, which seems to be a crucial step in the radial chromatography process.
  • 🌡 The use of a Caesar, presumably a tool or technique, is mentioned to create a streak on the filter, which is part of preparing the sample for chromatography.
  • 🕒 Time is an essential factor in the process, as the script talks about starting to time the process once the sample is on the filter.
  • 📏 The script mentions checking the process by placing the sample in front of a female, which seems to be a metaphor for a specific step or condition in the chromatography.
  • 📉 The process involves cutting the excess part of the sample to ensure a proper fit on the filter paper, which is a step towards achieving a complete garden, a term possibly referring to a complete separation of components.
  • 📝 The script also talks about entering something on Twitter, which might be a metaphor for recording or documenting the process of radial chromatography.
  • 📈 After the sample is prepared, the script mentions a process of mixing, which seems to involve a combination of blue green and red, possibly referring to different chemical components.
  • 📌 The script ends with a discussion about placing the mixture on a platform and ensuring a proper profit, which could be interpreted as achieving the desired outcome in the radial chromatography experiment.
Q & A

  • What is the purpose of radial chromatography mentioned in the script?

    -The script discusses the use of radial chromatography, a technique used for separating different components in a mixture, such as in the process of purifying a sample.

  • What is the first step in performing radial chromatography as described in the script?

    -The first step involves placing the sample on a completely round filter paper, which is then cut and placed in the exact center of a cold field.

  • What is the role of a Caesar in the process described?

    -The script metaphorically uses 'Caesar' to refer to a tool or method that can be used in the process, possibly indicating a technique or a piece of equipment that helps in the separation or purification process.

  • What is the significance of preparing a 'naavik' in the script?

    -The term 'naavik' is not clearly defined in the script, but it seems to refer to a preparation step in the process, possibly related to setting up the radial chromatography apparatus or preparing the sample.

  • How does the script describe the process of checking the progress of the chromatography?

    -The script suggests checking the progress by looking at the 'barik' side, which could be a reference to observing the separation or movement of the components on the filter paper.

  • What does the script mention about the final step after performing radial chromatography?

    -The final step mentioned involves placing the filter paper with the separated components onto a mobile phase, which helps in further separation and analysis of the components.

  • What is the importance of the 'mobile face' in the script?

    -The 'mobile face' seems to refer to a component or aspect of the radial chromatography process that is crucial for the movement or separation of the sample components.

  • How does the script describe the interaction between the sample and the 'mobile face'?

    -The script suggests that after placing the sample on the 'mobile face', one should observe the interaction, which could involve the components moving or separating due to the properties of the mobile phase.

  • What is the role of 'debbit 2m' in the script?

    -The term 'debbit 2m' is not clearly defined, but it seems to refer to a variable aspect of the process, possibly related to the amount of mobile phase used or the duration of the process.

  • What does the script suggest about the flexibility in the amount of mobile phase used?

    -The script indicates that there is flexibility in the amount of mobile phase used, as it states 'there is no limit to the quantity of mobile phase you can buy in the petri dish'.

  • What is the final outcome expected after completing the radial chromatography process as described in the script?

    -The final outcome is the separation of the components of the sample, with the script suggesting that the components will start to soften and separate, indicating successful chromatography.

Outlines
00:00
🔬 रेडियल क्रोमैटोग्राफी की प्रक्रिया

इस खंड में रेडियल क्रोमैटोग्राफी की प्रक्रिया का वर्णन दिया गया है। यह विषय शुरु होता है रेडियोग्राफी के साथ, जिसे अमृतसर में किसी भी कंप्लेंट के लिए इस्तेमाल किया जाता है। विवरण में रेलकर्मी डोगरा का उपयोग, फिल्टर काटने, नाविक तैयार करने, और मिश्रण के विभिन्न चरणों को समझाया गया है।

Mindmap
Keywords
💡Radial Chromatography
Radial chromatography is a technique used to separate different components of a mixture. In the video, it is demonstrated how to perform radial chromatography, focusing on the method and materials required for the process. This technique is essential for analyzing and identifying various compounds within a sample.
💡First Impression
The term 'first impression' refers to the initial evaluation or observation of different components within a mixture. In the context of the video, it is about the initial results observed when performing radial chromatography on a sample, crucial for understanding the composition of the mixture.
💡Filter Paper
Filter paper is a porous paper used to separate solids from liquids. In the video, it is used as a medium to place the sample mixture for radial chromatography. The filter paper helps in the separation process by allowing the solvent to carry different components at different rates.
💡Sample Mixture
A sample mixture is a combination of different substances that need to be separated and analyzed. In the video, a mixture of blue, green, and red components is used to demonstrate the radial chromatography process. Understanding the sample mixture is crucial for performing and interpreting chromatography results.
💡Mobile Phase
The mobile phase is the solvent that moves through the chromatography medium, carrying the different components of the sample with it. In the video, the mobile phase helps in separating the mixture components based on their interactions with the filter paper and the solvent.
💡Separation Process
The separation process involves dividing the components of a mixture based on their chemical properties. In radial chromatography, this is achieved by the movement of the mobile phase through the filter paper. The video explains how different components move at different rates, leading to their separation.
💡Spotting
Spotting refers to the application of small amounts of the sample mixture onto the chromatography medium. In the video, precise spots of the blue, green, and red mixture are placed on the filter paper to initiate the chromatography process. Accurate spotting is vital for obtaining clear separation results.
💡Concentration
Concentration in chromatography refers to the amount of a particular component within the sample mixture. The video shows how different concentrations of the components affect their separation during the chromatography process. Higher concentrations can lead to more distinct spots on the filter paper.
💡Solvent Movement
Solvent movement is the flow of the mobile phase through the chromatography medium. The video highlights how the movement of the solvent carries the components of the mixture, leading to their separation based on their affinities towards the solvent and the filter paper.
💡Visualization
Visualization in chromatography is the process of observing and interpreting the separated components on the filter paper. The video demonstrates how the separated spots of blue, green, and red components can be seen after the chromatography process, helping to identify and analyze the mixture.
Highlights

Introduction to radial chromatography and its application in separating different components.

Explanation of the first impression and the use of radial chromatography in Amritsar.

The process of setting up the initial filter bag on a completely spherical fielder.

Instructions on cutting and chilling the filter to prepare for sample collection.

Use of a Caesar to create a sieve in the mixture for radial chromatography.

Preparation of the navik (navigator) for recording the process.

Technique to check the setup by placing it in front of a female subject.

Method of cutting the excess part after the don (placing) process.

Description of the next steps after cutting, including examining the sample side.

Process of mixing the sample with a mobile face to create a complete mixture.

Instructions on placing the sample mixture on a platform for further processing.

Technique of pressing and sending the mobile for a minor adjustment.

Explanation of the solvent shooting process into the mobile face.

Details on setting the complete with debit 2m according to preference.

Process of preparing the filter paper and placing it on the mobile.

Technique of creating and speeding up the mobile face to start smiling.

Final steps in the radial chromatography process, including the separation of components.

Advantage of radial chromatography in being able to handle any company's product.

Transcripts

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