Using Serological Pipets

Bio-Rad Laboratories
16 Oct 201204:45
EducationalLearning
32 Likes 10 Comments

TLDRThis video tutorial guides viewers on utilizing serological pipets and different types of pipette pumps for measuring and transferring volumes over 1 milliliter. It covers the use of tri-valve pumps with their A, S, and E valves for filling and dispensing liquids, syringe-style pumps featuring a gasket, exhaust valve, dial, and plunger, and electronic pumps with a gasket, filter, suction, and exhaust buttons. Each method is explained step-by-step, ensuring precise liquid handling and minimizing contamination.

Takeaways
  • 🧪 Serological pipets are graduated pipets used for measuring volumes over 1 milliliter.
  • 📏 They have two scales, one for filling and one for dispensing.
  • 🔌 Serological pipets often come with a cotton plug to prevent contamination.
  • 🔄 Three main types of pipette pumps are used with serological pipets: tri-valve, syringe, and electronic pumps.
  • 🌀 The tri-valve pump has an air valve (A), a suction valve (S), and an exhaust valve (E) for filling and emptying the pipette.
  • 💉 To use a tri-valve pump, attach it to the pipette, squeeze the bulb to release air, press the S valve to fill, and the E valve to dispense.
  • 💧 The syringe-style pump includes a gasket, exhaust valve, dial for adjusting volume, and a plunger for expelling solution.
  • 🔄 With a syringe pump, insert the pipette into the gasket, roll the dial to suck up solution, and press the exhaust valve to transfer.
  • 🔋 The electronic pump features a gasket, filter, suction button, and exhaust button for precise volume control.
  • 🔗 Attach a pipette to an electronic pump by pushing and twisting it into place, then use the buttons to fill and dispense the solution.
  • 🚱 Any remaining solution in the pipette can be blown out using the appropriate features of the pump being used.
Q & A

  • What is a serological pipet used for?

    -A serological pipet is a type of graduated pipet commonly used to measure volumes over 1 milliliter.

  • What are the two scales on a serological pipet for?

    -Serological pipets have two scales: one for filling and the other for dispensing.

  • Why do serological pipets often have a cotton plug at one end?

    -The cotton plug is used to prevent contaminants from being drawn into the vacuum created by the pipet.

  • What are the three main types of pipet pumps mentioned in the script?

    -The three main types of pipet pumps mentioned are the tri-valve pump, the syringe pump, and the electronic pump.

  • How does the tri-valve pump work?

    -The tri-valve pump has three valves: an A or air valve to release air, an S or suction valve to fill the pipet, and an E or exhaust valve to empty any remaining liquid.

  • How do you fill a serological pipet using a tri-valve pump?

    -To fill the pipet, attach the pump to the pipet, squeeze the bulb to release air through the air valve, press the suction valve to fill the pipet, and then squeeze the exhaust valve until the meniscus touches the desired volume line.

  • What is the purpose of the gasket in a syringe style pipet pump?

    -The gasket in a syringe style pipet pump attaches to the pipet, ensuring a tight seal for accurate liquid transfer.

  • How do you adjust the amount of solution transferred using a syringe style pipet pump?

    -You use the dial on the syringe style pipet pump to adjust the amount of solution transferred.

  • What does the filter in an electronic pipet pump prevent?

    -The filter in an electronic pipet pump prevents any contaminants from getting into the pump.

  • How do you attach a pipet to an electronic pipet pump?

    -To attach a pipet to an electronic pump, firmly push and twist it into the gasket.

  • What buttons are used on an electronic pipet pump to obtain the exact volume of liquid?

    -The suction button and the exhaust button are used on an electronic pipet pump to obtain the exact volume of liquid.

  • How do you transfer the solution from a pipet to a target container using an electronic pipet pump?

    -Place the pipet tip into the solution, press the suction button to fill to the desired level, move the pipet to the target container, and slowly press the exhaust button until all of the solution has been transferred.

Outlines
00:00
🧪 Serological Pipettes and Pipette Pumps Overview

This paragraph introduces serological pipettes, which are graduated pipettes used for measuring volumes over 1 milliliter. It explains their dual scales for filling and dispensing, and the presence of a cotton plug to prevent contamination. The paragraph also outlines three main types of pipette pumps: the tri-valve, syringe, and electronic pumps, each with its specific components and functions.

Mindmap
Keywords
💡Serological Pipets
Serological pipets are a type of graduated pipet designed for measuring and transferring volumes of liquid over 1 milliliter. They are crucial in laboratory settings for precise volume measurements. In the video, serological pipets are described as having two scales for filling and dispensing, and a cotton plug to prevent contamination, emphasizing their importance in maintaining sterility and accuracy in liquid handling.
💡Pipet Pumps
Pipet pumps are devices used in conjunction with serological pipets to control the transfer of liquids. They come in different types, such as tri-valve, syringe, and electronic pumps. The video script explains that these pumps are essential for operating serological pipets, allowing for the precise filling and dispensing of liquid samples.
💡Tri-Valve Pump
A tri-valve pump is a specific type of pipet pump with three valves: an A or air valve, an S or suction valve, and an E or exhaust valve. The tri-valve pump is highlighted in the video for its role in releasing air, filling the pipet, and emptying any remaining liquid. The script provides a step-by-step guide on how to use this pump with a serological pipet, illustrating its practical application in liquid handling.
💡Meniscus
The meniscus refers to the curved surface of a liquid in a container, which is often observed when using a pipet. In the context of the video, the meniscus is important for determining the exact volume of liquid in the pipet, as the bottom of the meniscus should align with the desired graduated line on the pipet.
💡Syringe Style Pipet Pump
A syringe style pipet pump is another type of pump mentioned in the video script. It includes a gasket, exhaust valve, dial for adjusting the volume, and a plunger. This pump is used to fill and empty the pipet with precision, and the script describes its parts and functions, showing how it contributes to accurate liquid transfers.
💡Electronic Pipet Pump
An electronic pipet pump is a modern device that automates the process of liquid transfer. The video script describes its components, such as the gasket, filter, suction button, and exhaust button, and explains how it attaches to the pipet and operates. This pump is highlighted for its ease of use and precision in transferring exact volumes of liquid.
💡Contamination
Contamination refers to the presence of unwanted substances or organisms that can affect the outcome of an experiment. In the video, the use of a cotton plug in serological pipets and filters in electronic pipet pumps is emphasized to prevent contamination, highlighting the importance of maintaining a sterile environment in laboratory procedures.
💡Volume Measurement
Volume measurement is a fundamental aspect of laboratory work, especially when using serological pipets. The video script discusses the importance of accurate volume measurement for successful experiments, and how different types of pipet pumps facilitate this process.
💡Vacuum Source
A vacuum source is a device that creates a vacuum to draw liquid into a pipet. In the context of the video, the vacuum source is created by the action of the pipet pumps, which is essential for filling the serological pipets with the desired volume of liquid.
💡Sterility
Sterility is the state of being free from living microorganisms, which is critical in laboratory settings to prevent unwanted reactions or growths. The video script mentions the cotton plug in serological pipets as a measure to maintain sterility by preventing contaminants from entering.
💡Liquid Transfer
Liquid transfer is the process of moving liquid from one container to another using a pipet. The video script provides detailed instructions on how to perform liquid transfer using various pipet pumps, emphasizing the precision and control required for accurate experimental results.
Highlights

Serological pipets are a type of graduated pipet commonly used to measure volumes over 1 milliliter.

Serological pipets have two scales, one for filling and one for dispensing.

They often have a cotton plug at one end to prevent contaminants from being drawn into the vacuum source.

Serological pipets are used with three main types of pipet pumps: the tri-valve, syringe, and electronic pump.

A tri-valve pump has three valves - an A valve to release air, an S valve to fill the pipet, and an E valve to empty the pipet.

To use a tri-valve pump, attach it to a serological pipet by twisting the top of the pipet into the bottom of the bulb.

Gently squeeze the bulb to release air through the one-way air valve.

Press on the S or suction valve to fill the pipet just above the desired volume.

Gently squeeze the E or exhaust valve until the meniscus touches the desired graduated line.

Any remaining solution can be blown out by holding the S valve and squeezing the bulb at the same time.

A syringe-style pipet pump includes a gasket, exhaust valve, dial to adjust the volume, and plunger to expel remaining solution.

Insert the pipet into the gasket by pressing and twisting firmly.

Use the dial to suck up the desired amount of solution.

Press the exhaust valve to transfer the contents of the pipet.

Any remaining solution can be blown out by pressing down on the plunger with your thumb.

An electronic pipet pump includes a gasket, filter, suction button, and exhaust button.

Attach the pipet to the pump by firmly pushing and twisting it into the gasket.

Press the suction button to fill the pipet to the desired level.

Use the suction and exhaust buttons to obtain the exact volume needed.

Slowly press the exhaust button to transfer all of the solution to the target container.

Transcripts

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Serological PipetsPipet PumpsLiquid MeasurementLab TechniquesTri Valve PumpSyringe PumpElectronic PumpContamination PreventionVolume ControlLaboratory SafetyScientific Tools