Two-way repeated measures ANOVA in SPSS: one-within, one-between (March 2020)

Mike Crowson
2 Mar 202021:17
EducationalLearning
32 Likes 10 Comments

TLDRThis video tutorial demonstrates how to perform a two-way repeated measures ANOVA in SPSS, focusing on scenarios with one between-subjects factor and one within-subjects factor. The presenter guides viewers through the process using an anxiety study example, explaining how to analyze data for significant mean differences over time and across treatment groups. The video also covers the interpretation of multivariate and univariate test results, assumptions checks, and the use of simple effects tests to explore interactions.

Takeaways
  • πŸ“š The video demonstrates how to conduct a two-way repeated measures ANOVA in SPSS, focusing on cases with one between-subjects factor and one within-subjects factor.
  • πŸ”— Links to the SPSS data file and a supplemental PowerPoint are provided in the video description for reference and follow-along.
  • πŸ“ˆ The example used in the video involves testing for significant mean differences in anxiety scores across three measurement occasions and between different treatment groups.
  • πŸ“Š The between-subjects factor is the treatment group, coded as 1 for control, 2 for treatment A, and 3 for treatment B.
  • πŸ“ The video covers the process of setting up the repeated measures analysis in SPSS, including defining the within-subject factor, measures, and between-subjects factor.
  • πŸ“‰ The script discusses the importance of checking for the assumption of sphericity in repeated measures ANOVA and how to interpret the results of Mauchly's test.
  • πŸ“‹ The video explains the output of the repeated measures ANOVA, including multivariate and univariate test results, and the implications of each.
  • πŸ“Š The results indicate a significant main effect of time and a significant interaction effect between time and treatment group on anxiety scores.
  • πŸ“ˆ The video describes how to interpret the profile plot, which shows the trend of anxiety scores over time and the differences between the treatment groups.
  • πŸ” The script also covers post-hoc tests, such as pairwise comparisons using Bonferroni adjustments, to further analyze the differences between time points and treatment groups.
  • πŸ› οΈ The video concludes with instructions on how to perform simple effects tests in SPSS to explore the nature of significant interactions.
Q & A
  • What is the main topic of the video?

    -The video demonstrates how to perform two-way repeated measures ANOVA in SPSS, focusing on scenarios with one between-subjects factor and one within-subjects factor.

  • What is the purpose of the supplemental PowerPoint mentioned in the video?

    -The supplemental PowerPoint provides additional information and visual aids to support the content being discussed in the video presentation.

  • Why is repeated measures ANOVA used in research?

    -Repeated measures ANOVA is used to analyze data where an outcome variable is measured repeatedly, often to test hypotheses about the variation in means across different groups over time.

  • What is the role of the 'between-subjects factor' in the study presented in the video?

    -In the study, the between-subjects factor is the 'treatment group', which is used to test if there are significant mean differences in anxiety scores across different treatment conditions over time.

  • How many measurement occasions are considered in the example provided in the video?

    -There are three measurement occasions considered in the example: Time 1, Time 2, and Time 3.

  • What statistical test is used to assess the main effect of time and the interaction effect in the video?

    -Multivariate tests and univariate tests are used to assess the main effect of time and the interaction effect between time and treatment group.

  • What does it mean if the multivariate test result is statistically significant?

    -A statistically significant multivariate test result suggests that the variances and covariances of different scores across the groups are significantly different, indicating a potential interaction effect.

  • What is the assumption of sphericity in the context of repeated measures ANOVA?

    -Sphericity is the assumption that the variances of the differences between all possible pairs of within-subject conditions are equal, which is important for the validity of the ANOVA results.

  • How does the video handle the potential issue of non-sphericity?

    -The video discusses using the Mauchly's test to assess sphericity and suggests using the Greenhouse-Geisser or Huynh-Feldt adjustments if the assumption of sphericity is violated.

  • What is the purpose of estimating marginal means in the video?

    -Estimating marginal means allows the researcher to understand the average scores across different conditions, irrespective of other variables, and to compare these means between different groups or time points.

  • What is the significance of the profile plot shown in the video?

    -The profile plot visually represents the interaction between time and treatment group, showing how the mean anxiety scores change over time for each treatment condition.

  • How does the video address the potential need for pairwise comparisons?

    -The video describes using Bonferroni adjustments for pairwise comparisons to account for multiple testing and to control the family-wise error rate.

  • What additional analysis is suggested by the video for further exploration of significant interactions?

    -The video suggests conducting simple effects tests to describe the nature of the interaction by examining mean differences within each level of the between-subjects factor.

Outlines
00:00
πŸ“Š Introduction to Two-Way Repeated Measures ANOVA in SPSS

This paragraph introduces the topic of the video, which is a tutorial on performing two-way repeated measures ANOVA using SPSS. The focus is on scenarios with one between-subjects factor and one within-subjects factor. The video also mentions the availability of a data file and a PowerPoint supplement for the audience's reference. The explanation begins with a review of repeated measures ANOVA and its application in comparing groups over time, leading to the hypothesis of interaction effects between the repeated factor and a grouping variable. The example used in the video involves testing anxiety scores across three time points for different treatment groups.

05:01
πŸ” Exploring Multivariate and Univariate Test Results

The second paragraph delves into the results of the multivariate and univariate tests from the ANOVA analysis. It discusses the implications of significant multivariate test results for the main effect of time and the interaction between time and treatment group. The paragraph also addresses the assumption of equality of variance-covariance matrices and the sensitivity of the Box's test to multivariate non-normality. The robustness of the multivariate test is considered in the context of equal or nearly equal group sizes, and the potential impact of non-normality and sample size on the validity of the test results is highlighted.

10:02
πŸ“‰ Analyzing Within-Subjects Effects and Trend Assessment

This section of the script examines the within-subjects effects, focusing on the main effect of time and the interaction between time and treatment group. It discusses the use of tests such as Mauchly's test for sphericity and the implications of its violation on the likelihood of type I errors. The script also explores the use of the Greenhouse-Geisser and Huynh-Feldt adjustments for tests of within-subjects effects, providing guidance on which tests to use based on the degree of departure from sphericity. The significance of linear and quadratic trends over time is also highlighted, with the suggestion that the data may follow a more complex, curvilinear pattern.

15:03
πŸ“ Tests of Between-Subjects Effects and Estimated Marginal Means

The fourth paragraph discusses the tests of between-subjects effects, which involve comparing the average anxiety scores across the different treatment groups. It notes the statistical significance of these tests, indicating differences in average anxiety levels among the groups. The paragraph also covers the estimated marginal means for anxiety at different time points, irrespective of group membership, and the pairwise comparisons that reveal significant differences between time points. The importance of considering the results of Levene's test for equality of error variances when interpreting these findings is emphasized.

20:06
πŸ“Š Simple Effects Tests and Describing Interactions

The final paragraph of the script outlines the process of conducting simple effects tests to further explore and describe the nature of interactions found in the analysis. It provides a step-by-step guide on modifying the SPSS syntax to request these tests, which involve comparing mean differences in anxiety over time within each treatment group. The results of these tests, including both multivariate and pairwise comparisons, are presented, showing significant mean differences within each group over time. The paragraph concludes by discussing how to compare groups within each time point, offering a comprehensive understanding of the interaction effects.

Mindmap
Keywords
πŸ’‘Two-way repeated measures ANOVA
A statistical test used to determine the effects of two independent variables on a dependent variable, where one variable is measured across multiple time points (within-subjects factor) and the other variable distinguishes different groups (between-subjects factor). This test is central to the video as it is the primary analysis demonstrated.
πŸ’‘Between-subjects factor
An independent variable in an experiment that categorizes participants into different groups. In the video, the between-subjects factor is the treatment group, with levels coded as control, treatment A, and treatment B, and it helps to compare anxiety scores across these groups.
πŸ’‘Within-subjects factor
An independent variable in an experiment where the same participants are measured across multiple time points. In the video, time is the within-subjects factor, representing anxiety measurements taken at three different occasions (time 1, time 2, time 3).
πŸ’‘SPSS
A software package used for statistical analysis. The video provides a demonstration of how to perform a two-way repeated measures ANOVA using SPSS, highlighting its features and steps required for this analysis.
πŸ’‘Interaction effect
Occurs when the effect of one independent variable on the dependent variable depends on the level of another independent variable. The video explores the interaction effect between the time of anxiety measurement and the treatment group, examining how these two factors interact to influence anxiety scores.
πŸ’‘Estimated marginal means
Means adjusted for the effects of other variables in the model. In the video, these means are calculated to understand the average anxiety scores across different times and treatment groups, facilitating pairwise comparisons.
πŸ’‘Pairwise comparisons
Statistical comparisons between different levels of an independent variable. In the video, pairwise comparisons are made between different time points and treatment groups to identify significant differences in anxiety scores.
πŸ’‘Multivariate tests
Statistical tests that consider multiple dependent variables simultaneously. The video explains how multivariate tests, such as Wilks' lambda, are used to assess the main effects and interactions in a two-way repeated measures ANOVA.
πŸ’‘Sphericity
An assumption in repeated measures ANOVA that the variances of the differences between all combinations of related groups are equal. The video discusses how violations of sphericity can impact test results and how adjustments like Greenhouse-Geisser are used to correct for such violations.
πŸ’‘Greenhouse-Geisser adjustment
A statistical correction applied when the assumption of sphericity is violated in repeated measures ANOVA. The video illustrates the use of the Greenhouse-Geisser adjustment to provide more accurate p-values when evaluating within-subject effects.
Highlights

Introduction to two-way repeated measures ANOVA with one between-subjects factor and one within-subjects factor using SPSS.

Availability of the SPSS data file and supplemental PowerPoint for download.

Explanation of repeated measures ANOVA for more than a single group and its application in testing hypotheses about interaction effects.

Use case scenario where researchers test whether differences in means over time are consistent across levels of a grouping variable.

Coding of the between-subjects factor with three levels: control, treatment A, and treatment B.

Objective to test for significant mean differences in anxiety scores over three time points and differences in trends over time across groups.

Demonstration of how to set up the repeated measures analysis in SPSS, including defining within and between-subjects factors.

Inclusion of descriptive statistics, effect size, power, and homogeneity tests in the analysis.

Use of estimated marginal means and pairwise comparisons with Bonferroni adjustments for multiple testing.

Interpretation of multivariate test results for main effects and interaction effects, including the significance of Wilks lambda.

Discussion on the robustness of multivariate test results and the sensitivity of Box's test to multivariate non-normality.

Assessment of sphericity using Mauchly's test and alternatives like Greenhouse-Geisser and Huynh-Feldt adjustments.

Guidance on choosing the appropriate tests based on the degree of sphericity violation using epsilon values.

Analysis of within-subjects contrasts to assess trends over time irrespective of group membership.

Identification of significant linear and quadratic trends in anxiety scores over time from the profile plot.

Investigation of differential trending across treatment groups using interaction effects for linear and quadratic components.

Tests of between-subjects effects to determine if there are significant differences in average anxiety scores across groups.

Presentation of estimated marginal means for anxiety by time point and pairwise comparisons between time points.

Pairwise comparisons of anxiety scores between different treatment groups and their statistical significance.

Introduction to simple effects tests for describing the nature of significant interactions between factors.

Demonstration of how to modify SPSS syntax for simple effects tests and interpretation of the results.

Transcripts
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