How to pipette correctly – a short step-by-step introduction into proper pipetting

Eppendorf
27 Feb 201703:09
EducationalLearning
32 Likes 10 Comments

TLDRThis tutorial offers a step-by-step guide to accurate pipetting techniques using a 2-button pipette. It emphasizes the importance of a vertical aspiration angle, minimal immersion depth, and gentle tip attachment to avoid strain and damage. The script details the process of aspirating and dispensing liquid, including adjusting the pipette angle to 20-45 degrees for optimal flow and ensuring complete tip ejection into a garbage bin for reliable results.

Takeaways
  • 🔬 Use a 2-button pipette with a control button for precise liquid handling.
  • 💧 Gently attach the pipette tip without force to avoid damage to the pipette.
  • ⚖️ Two factors, aspiration angle and immersion depth, are crucial for accurate liquid uptake.
  • 📐 Maintain a vertical aspiration angle to prevent over-aspiration.
  • 🌊 Keep immersion depth minimal but sufficient to avoid capillary effect issues.
  • 🔄 Press the control button to the first stop and submerge the tip to aspirate liquid.
  • 🔁 Repeat the aspiration process two to three times to properly situate air in the tip.
  • 📈 Adjust the pipette angle to 20-45 degrees for dispensing liquid while in contact with the vessel wall.
  • 💨 Press the control button to the first stop and then to the second stop to ensure complete liquid ejection.
  • 🗑️ Dispose of the used pipette tip by pressing the ejector button into a designated garbage bin.
  • 🛠️ Improve pipetting skills by mastering soft tip attachment, vertical aspiration, low immersion depth, and the correct dispensing angle.
Q & A

  • What is the main topic of the video tutorial?

    -The main topic of the video tutorial is the correct method of using a 2-button pipette for accurate and reliable pipetting.

  • What are the two functions of the control button on a 2-button pipette?

    -The control button on a 2-button pipette has two functions: aspirating liquid to the first stop and ejecting the tip.

  • How should you attach the tip to the pipette?

    -When attaching the tip, apply light pressure to insert the pipette into the tip without using force or moving from side to side to avoid straining your arm or damaging the pipette.

  • What are the two factors that influence accurate liquid uptake when using a pipette?

    -The two factors that influence accurate liquid uptake are the aspiration angle and the immersion depth of the pipette tip in the liquid.

  • Why should the aspiration angle be vertical when using a pipette?

    -The aspiration angle should be vertical to prevent aspirating too much liquid, which can happen if the angle is not straight.

  • What does the immersion depth refer to when pipetting?

    -The immersion depth refers to the depth of the pipette tip inside the liquid, which should be as large as necessary but as small as possible to avoid exploiting additional liquid due to the capillary effect.

  • How many times should you press and release the control button to ensure proper air positioning in the tip?

    -You should press the control button down to the first stop and release it two to three times to ensure proper air positioning in the tip.

  • What is the recommended angle for the pipette when expecting liquid?

    -The recommended angle for the pipette when expecting liquid is between 20 and 45 degrees, ensuring optimal flow out of the liquid.

  • Why should the pipette tip stay in contact with the vessel wall when dispensing liquid?

    -The pipette tip should stay in contact with the vessel wall to guarantee optimal flow and prevent splashing or inaccurate dispensing.

  • How do you ensure that the pipette tip is completely emptied after dispensing liquid?

    -To ensure the pipette tip is completely emptied, press the control button down to the first stop, then press further down to the second stop to blow out any remaining liquid.

  • What is the final step in the pipetting process as described in the tutorial?

    -The final step in the pipetting process is ejecting the used tip into a garbage bin by pressing the ejector button.

Outlines
00:00
🧪 Pipetting Technique Basics

This paragraph introduces the fundamentals of pipetting in a laboratory setting. It covers the use of a 2-button pipette with a control button and an ejector mechanism. The importance of proper pipette tip attachment is emphasized, with a light pressure technique to avoid arm strain or pipette damage. The paragraph also explains the factors influencing accurate liquid uptake, such as aspiration angle and immersion depth, and provides a step-by-step guide for aspirating and dispensing liquids correctly. The correct angle for pipetting (20 to 45 degrees) and the method for tip ejection into a garbage bin are also included.

Mindmap
Keywords
💡Packaging Tutorial
A 'Packaging Tutorial' refers to an instructional video or guide that teaches viewers how to properly package or handle items, in this case, liquids using a pipette. It is the central theme of the video, focusing on the correct technique for pipetting to ensure accurate and reliable results. The tutorial provides step-by-step guidance on how to use a pipette correctly.
💡Pipette
A 'Pipette' is a laboratory tool used to transfer or measure out small volumes of liquid. In the script, it is the main subject of the tutorial, with a focus on a '2 button pipette' that has specific features for controlling the amount of liquid aspirated and dispensed. The pipette is essential for accurate liquid handling in various scientific applications.
💡Control Button
The 'Control Button' on a pipette is used to aspirate or dispense liquid. The script mentions it has 'two stops,' which likely refer to different levels of depression to control the volume of liquid being moved. This button is crucial for the pipetting process, allowing for precise volume adjustments.
💡Ejector Button
The 'Ejector Button' is used to release the pipette tip after use. In the script, it is mentioned as part of the final step in the pipetting process, ensuring that the used tip is ejected into a garbage bin for proper disposal, maintaining hygiene in the laboratory setting.
💡Aspiration Angle
The 'Aspiration Angle' is the angle at which the pipette tip is inserted into the liquid to draw it up. The script emphasizes that it should be vertical to avoid aspirating too much liquid. This term is important for understanding how to achieve accurate liquid uptake.
💡Immersion Depth
'Immersion Depth' refers to how far the pipette tip is inserted into the liquid. The script advises keeping it as small as possible but as large as necessary to prevent additional liquid from being aspirated due to capillary action. This concept is vital for precision in liquid handling.
💡Capillary Effect
The 'Capillary Effect' is a phenomenon where a liquid flows through narrow spaces without the assistance of external forces, such as in a pipette tip. The script warns against immersing the tip too deeply to avoid exploiting this effect, which could lead to inaccurate measurements.
💡Dispensing Angle
The 'Dispensing Angle' is the angle at which the pipette is held when dispensing liquid. The script specifies it should be between 20 and 45 degrees while in contact with the vessel wall to ensure optimal flow. This angle is critical for the accurate dispensing of the liquid.
💡Vessel Wall
The 'Vessel Wall' refers to the side of the container holding the liquid. In the context of the script, the pipette tip should stay in contact with the vessel wall when dispensing liquid to ensure a smooth and accurate transfer. This detail is important for maintaining control over the pipetting process.
💡Soft Tip Attachment
'Soft Tip Attachment' likely refers to the method of attaching the pipette tips, which are often made of soft plastic to ensure a secure fit and prevent leakage. The script mentions this as part of achieving accurate pipetting results, indicating the importance of proper tip attachment.
💡Vertical Aspiration
'Vertical Aspiration' is the method of aspirating liquid with the pipette held vertically, as advised in the script to ensure accurate liquid uptake. This technique is fundamental to the pipetting process and is emphasized for its importance in achieving reliable results.
Highlights

Introduction to a packaging tutorial focusing on pipetting techniques.

Use of a 2-button pipette with a control button and ejector mechanism.

Proper attachment of the pipette tip with light pressure to avoid damage.

Importance of a vertical aspiration angle for accurate liquid uptake.

Optimal immersion depth to prevent additional liquid aspiration.

Technique of pressing the control button to the first stop for liquid aspiration.

Repetition of the aspiration technique for proper air positioning in the tip.

Angle of pipette during liquid dispensing should be between 20 to 45 degrees.

Maintaining tip contact with the vessel wall for optimal liquid flow.

Pressing the control button to the first stop and then blowing out for complete emptying.

The final step of ejecting the used tip into a garbage bin.

Achieving accurate pipetting with soft tip attachment and vertical aspiration.

Low immersion depth as a key to reliable pipetting results.

20 to 45-degree dispensing angle in direct contact with the vessel wall for improved packaging skills.

The conclusion emphasizing the improvement of packaging skills through proper pipetting techniques.

Transcripts

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Related Tags
Pipetting TutorialLab TechniquesLiquid HandlingControl ButtonAspiration AngleImmersion DepthAccurate DispensingTip AttachmentLab SafetyVertical AspirationDispensing Angle