How to Clean Analytical Glassware

Wits University OFFICIAL
30 Jul 201803:46
EducationalLearning
32 Likes 10 Comments

TLDRThis instructional video outlines the crucial steps for cleaning analytical glassware to ensure accurate experimental results, particularly for titrations. It details the cleaning process for a burette and pipette, emphasizing the importance of using a cleaning solution and distilled water for thorough rinsing. The video guides viewers through the steps of filling the burette with tap water, flushing it with the cleaning solution, and rinsing it with distilled water. For pipettes, it covers attaching the tip, cleaning with a diluted solution, and rinsing under running water until water droplets no longer adhere to the inner surface. The video concludes by stressing the necessity of clean glassware to avoid contamination and errors in experiments, offering assistance from demonstrators for any cleaning challenges.

Takeaways
  • 🔬 Cleaning apparatus is crucial for accurate experimental results, particularly in analytical chemistry.
  • 🧪 The three main pieces of apparatus used in titrations are the burette, pipette, and conical flask.
  • 💧 Cleaning a burette involves using a cleaning solution or detergent, followed by rinsing with tap water and distilled water.
  • 🚰 The burette should be filled with tap water and then some of the cleaning solution should run into the tip.
  • 💦 After cleaning, the burette should be rinsed thoroughly with tap water to ensure no residue remains.
  • 🔍 The pipette must be fitted with an appropriate pipette tip and cleaned by sucking up a diluted cleaning solution.
  • ⏳ The pipette cleaning process includes rinsing under a gentle running tap for about a minute until water droplets do not stick to the inner surface.
  • 💧 Distilled water is used to rinse the pipette to remove any remaining tap water after cleaning.
  • 🛠️ If problems arise with the pipette, it should be removed and dried with a paper towel before reuse.
  • 👩‍🔬 It is important to ask a demonstrator for assistance if there are any issues during the cleaning process.
Q & A

  • Why is it important to clean glassware in analytical experiments?

    -Cleaning glassware is crucial in analytical experiments to ensure contaminant-free apparatus, which is essential for obtaining accurate results and avoiding errors caused by contamination.

  • What are the three main pieces of apparatus required for titrations?

    -The three main pieces of apparatus required for titrations are the burette, pipette, and conical flask.

  • Why is cleaning the burette and pipette not a straightforward task?

    -Cleaning the burette and pipette is not straightforward due to their narrow shape and the pivot, which makes it difficult to clean them properly without the correct method.

  • How should a 50ml burette be cleaned according to the script?

    -A 50ml burette should be cleaned by adding approximately 20ml of cleaning solution, filling it with tap water, allowing some solution to run into the tip, rinsing it thoroughly with tap water five to six times, and then rinsing with distilled water from a wash bottle.

  • What should be checked after cleaning the burette to ensure it is functioning properly?

    -After cleaning the burette, it should be checked to ensure the tip is loose enough to turn but tight enough to prevent any leakages.

  • How is the pipette fitted with the appropriate pipette tip?

    -The pipette is fitted with the appropriate pipette tip by loosening the white end, inserting the tip end into the pipette about 2 centimeters, and then tightening the white end using a gentle twisting motion.

  • What is the cleaning procedure for the pipette according to the script?

    -The pipette is cleaned by sucking up diluted cleaning solution approximately two to three centimeters above the graduation line, running the solution out into the sink, and repeating the process two more times. It is then rinsed with distilled water and checked for cleanliness by ensuring water droplets do not stick to the inner surface.

  • What should be done if the cleaning solution is sucked up into the pipette?

    -If the cleaning solution is sucked up into the pipette, the pipette should be removed and dried using a paper towel before being reused.

  • Why is it necessary to rinse the pipette with distilled water after cleaning?

    -Rinsing the pipette with distilled water after cleaning is necessary to remove any remaining tap water and ensure that the pipette is completely clean for accurate measurements.

  • What should you do if you encounter any problems with your burette or pipette during cleaning?

    -If there are any problems with your burette or pipette during cleaning, you should ask your demonstrator for assistance.

Outlines
00:00
🧪 Proper Cleaning Techniques for Analytical Glassware

This paragraph emphasizes the importance of using contaminant-free apparatus for accurate experimental results, particularly in analytical investigations like titrations. It outlines the three main pieces of apparatus required for titrations: the burette, tippet, and conical flask. The paragraph provides a step-by-step guide on cleaning a 50ml burette, which includes adding cleaning solution, rinsing with tap water, and final rinsing with distilled water. It also covers the cleaning process for a pipette, detailing the fitting of the pipette tip, dilution and use of cleaning solution, rinsing with tap water, and final rinsing with distilled water. The paragraph concludes with a reminder to ensure all glassware is clean to avoid contamination errors and offers assistance from a demonstrator if needed.

Mindmap
Keywords
💡Analytical class
Analytical class refers to a type of laboratory class where analytical chemistry techniques are taught and practiced. In the context of the video, it is where students learn how to clean laboratory glassware to ensure accurate results in their experiments. The term is used to emphasize the importance of cleanliness in obtaining precise measurements and avoiding contamination.
💡Contaminant-free apparatus
A contaminant-free apparatus is essential for conducting accurate experiments, as it refers to laboratory equipment that is free from any substances that could interfere with the results. In the video, the script highlights the importance of using clean equipment, such as burettes and pipettes, to prevent any contamination that might affect the outcome of analytical investigations.
💡Titration
Titration is a laboratory technique used to determine the concentration of an unknown solution by reacting it with a solution of known concentration. The video discusses the necessity of having clean apparatus for titration, as it is an analytical investigation that requires precision. The script mentions titration to illustrate the practical application of clean glassware.
💡Burette
A burette is a piece of laboratory equipment used to deliver a precise volume of liquid during titration. The script provides detailed instructions on how to clean a burette, emphasizing the importance of this step in ensuring the accuracy of titration results. The burette is a key component of the apparatus discussed in the video.
💡Pipette
A pipette is a laboratory tool used to transfer measured volumes of liquid. The video script outlines the steps to clean a pipette, which is crucial for maintaining the integrity of the experiments. The pipette is highlighted as another essential piece of apparatus that must be kept clean to avoid errors in analytical chemistry.
💡Conical flask
A conical flask is a type of laboratory glassware with a narrow top and a wider bottom, often used to hold solutions during experiments like titration. Although not explicitly detailed in the cleaning process, the conical flask is mentioned as one of the three main pieces of apparatus required for titrations, implying its importance in maintaining a contaminant-free environment.
💡Cleaning solution
A cleaning solution or detergent is a substance used to clean laboratory glassware by removing contaminants and residues. The video script describes the process of using a cleaning solution to clean the burette and pipette, which is a critical step in the preparation of apparatus for accurate experimental results.
💡Distilled water
Distilled water is water that has been purified through distillation, removing impurities and minerals. In the video, distilled water is used to rinse the glassware after cleaning with a detergent, ensuring that no residue from the tap water remains. This step is crucial for maintaining the purity of the apparatus.
💡Detergent
Detergent is a cleaning agent that helps to remove dirt and stains. The script mentions the use of detergent as part of the cleaning solution for the glassware. Detergents are important in the cleaning process as they break down and remove contaminants effectively.
💡Demonstrator
A demonstrator in a laboratory setting is an individual who assists students with practical aspects of experiments, providing guidance and help when needed. The video script suggests asking a demonstrator for assistance if there are any problems with the apparatus, highlighting the role of demonstrators in facilitating a successful and accurate laboratory experience.
💡Contamination
Contamination refers to the presence of impurities or unwanted substances that can affect the outcome of an experiment. The video emphasizes the need to avoid contamination by thoroughly cleaning all glassware. The term is used to stress the importance of cleanliness in achieving accurate and reliable results in analytical chemistry.
Highlights

Importance of clean apparatus for accurate experimental results

Emphasis on cleanliness in analytical investigations like titration

Three main apparatus for titration: buret, tippet, and conical flask

Challenges in cleaning due to the shape of glassware

Correct method for cleaning glassware in practicals

Cleaning the 50ml burette with cleaning solution and water

Filling the burette with tap water and rinsing with tap water

Ensuring no water droplet sticks to the inner surface of the burette

Final rinse of the burette with distilled water

Checking the burette tap for proper tightness and looseness

Fitting the pipette with an appropriate pipette tip

Diluting cleaning solution in a beaker for pipette cleaning

Cleaning the pipette by sucking up and running out the solution

Removing the pipette tip and rinsing under running water

Inspecting the pipette for cleanliness by water droplet adherence

Rinsing the pipette with distilled water to remove tap water

Advice on seeking assistance if there are issues with the apparatus

The necessity of clean glassware to avoid contamination errors

Transcripts

Browse More Related Video

How to Prepare a Burette for a Titration

How to use a burette

Titration Video

Burettes

What Happens During a Deep Cleaning Procedure (Scaling and Root Planing)

How to Use & Clean an Analytical Balance

Rate This

5.0 / 5 (0 votes)

Thanks for rating:

Related Tags
Glassware CleaningAnalytical ChemistryLab TechniquesTitration ProcessContamination ControlLaboratory SafetyBurette CarePipette UsageCleaning SolutionsDistilled Water